Perspective Chapter: Recent Progressions on the Colorimetric Diagnosis of SARS-CoV-2 by Loop-Mediated Isothermal Amplification (LAMP) Assay

A simple, fast, and accurate diagnosis of SARS-CoV-2 is great importance for the patient’s isolation, treatment, control COVID-19 pandemic. Although RT-qPCR accepted as gold standard, studies to improve more reliable diagnostic methods are continuing. Colorimetric reverse transcription loop-mediated isothermal amplification (RT-LAMP) a method that allows visual detection without needing expensive fluorescence readers. However, performance assay depends on some factors, such selection target gene (i.e., N, RdRp, S, E, M), primer design, dye used observation—neutral red, calcein, cresol or phenol red—and reaction conditions buffer pH, temperature, enzyme concentration. In last 2 years, plenty research has been conducted obtain best performance. this chapter, recent progressions colorimetric RT-LAMP comprehensively elucidated.